COMPARISON OF TWO BIOMODELS (BALB/C MICE AND SPRAGUE DAWLEY RATS) IN THE ALKALINE COMET ASSAY

Contenido principal del artículo

Daniel Francisco Arencibia Arrebola
Luis Alfredo Rosario Fernández
Yolanda Emilia Suárez Fernández
Alexis Vidal Novoa

Resumen

This article had as objective to carry out a comparison between Balb/c mice and Sprague Dawley rats as biomodel in the alkaline comet assay, keeping in mind the basal frequency and that induced with Cyclophosphamide, the induction of single strand breaks (SSB) or alkali-labile sites formation on DNA of peripheral blood leukocytes. Ten animal/sex/species/group of Balb/c mice and Sprague Dawley rats were used, and treated for 14 days. Using a negative control group (not treated), two substance-vehicle controls and a positive control received cyclophosphamide 50 mg/kg, via intraperitoneal. After two weeks the alkaline electrophoresis gel was performed for individual cells from leukocytes of peripheral blood to screen for possible DNA damage. The best biomodel in both sexes was the SD rats differing significantly with the results obtained in Balb/c mice keeping in mind the spontaneous and induced values of DNA damage. This study shows SD rats are a more efficient model for preclinical genotoxic evaluation of drugs, vaccines and natural products.

Detalles del artículo

Cómo citar
Arencibia Arrebola, D. F., Rosario Fernández, L. A., Suárez Fernández, Y. E., & Vidal Novoa, A. (2020). COMPARISON OF TWO BIOMODELS (BALB/C MICE AND SPRAGUE DAWLEY RATS) IN THE ALKALINE COMET ASSAY. BIOCYT Biología Ciencia Y Tecnología, 4(13-16). https://doi.org/10.22201/fesi.20072082.2011.4.75943

Citas en Dimensions Service