Effect of different colored light on motor activity and adult emergence in Tenebrio molitor pupae Effect of light on Tenebrio molitor pupae
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Abstract
Light is a factor that influences the physiology and behavior of insects. Although pupae have traditionally been considered unreceptive to external stimuli, recent studies have shown that they are capable of detecting light signals. In this study, the effect of different wavelengths of light (red, green, blue, infrared, and white) on abdominal motor activity and the adult emergence rate in Tenebrio molitor pupae was evaluated. Five experimental groups of 12 pupae each were exposed to LED light for 14 minutes. Both abdominal movements and the proportion of individuals that reached adulthood were recorded. The results indicated that blue light, and to a greater extent, white light, elicited a significant motor response in T. molitor pupae (p < 0.001), demonstrating differential sensitivity to these wavelengths during the pupal stage. In contrast, no significant differences in emergence rate were observed between treatments (p = .441), suggesting that 14 minutes of exposure to LED sources does not affect adult development. These results show that T. molitor pupae perceive and integrate specific light stimuli. Furthermore, abdominal movements resulting from exposure to white and blue LEDs suggest that their use at this stage could be useful for investigating sensory integration during metamorphosis. Future studies could explore their potential for analyzing early cognitive processes in holometabolous insects.
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