Determinación de células T CD3+, CD4+, CD8+, receptor de la célula T familias VB en biopsias de tejido gingival en pacientes con periodontitis crónica

Abstract

The established periodontal lesions are characterized by BL, TL and macrophage dense infiltrations. Its important to determine new ways, as well as to evaluate and localize the cellular types in different sites in epithelial and connective tissues to establish the behavior between different sites in the same patient and between sites of different patients. Ten biopsies with chronic periodontitis, were evaluated for immunohistochemistry for anti CD4, CD8, CD3, Vβ2, Vβ5.1, Vβ6.7, Vβ8, Vβ12. The first stains shown an uniform behavior in the epithelial and connective tissue between patients, between different sites in the same patient and between sites of different patients, demonstrating an homogeneous behavior. For all the variables, only significant differences were observed for the kind of tissue showing a strong expression in the connective tissue. The greater TCR expression of the variable families evaluated were to Vβ6.7, followed by Vβ8, Vβ5. The expression for all the TCR markers shows significant differences when the analysis was individualized through the epithelial tissue and the connective tissue. Although, Vβ8 y Vβ5.1 were predominant for epithelial tissue, and Vβ6.7, Vβ8 for connective tissue. The TCR Vβ expression intrasite and intersite was variable. This finding was extremely interest because demonstrates a totally individual behavior which could indicate that the immune system responds of local manner.